ddm solubilization buffer (Thermo Fisher)

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Thermo Fisher ddm solubilization buffer
Ddm Solubilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+buffer+ddm/HEPES/pm39667931-297-4-22
Average 99 stars, based on 1 article reviews

ddm solubilization buffer - by Bioz Stars, 2026-11

99/100 stars

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Membrane:

Article Title: Parathyroid hormone senses extracellular calcium to modulate endocrine signaling upon binding to the family B GPCR parathyroid hormone 1 receptor
Article Snippet: .. The membrane pellet was solubilized with solubilization buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 5 mM CaCl 2 , 5 mM MgCl 2 , 2 mM EDTA, 10% glycerol and 0.5% n-Dodecyl-β-D-maltoside (DDM, ACROS Organics)). ..

Article Title: Parathyroid Hormone Senses Extracellular Calcium To Modulate Endocrine Signaling upon Binding to the Family B GPCR Parathyroid Hormone 1 Receptor.
Article Snippet: Parathyroid hormone (PTH) binds to a family B G protein coupled receptor, parathyroid hormone 1 receptor (PTH1R).. One of its functions is to regulate Ca homeostasis in bone remodeling, during which Ca can reach up to 40 mM.. A truncated version of PTH, PTH(1-34), can fully activate PTH1R and has been used for osteoporosis treatments.

Article Title: A tetrameric SpoVA2 membrane complex is required for DPA transport into Bacillus anthracis spores
Article Snippet: .. The membrane pellet was homogenized in solubilization buffer (20 mM HEPES at pH 7.6, 150 mM NaCl, 20% glycerol, 1% n-dodecyl-β-D-maltopyranoside [DDM; Thermo Fisher]) and rotated for 1 h at 4°C. ..

Expressing:

Article Title: Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
Article Snippet: .. Pellets from FMOR expressing HEK293 cells were solubilized in solubilization buffer [20 mM HEPES pH 7.5, 150 mM NaCl, 30% glycerol, 0.5% n-dodecyl-β-D-maltoside (DDM), 0.3% (3-((3-cholamidopropyl) dimethylammnonio)−1-propanesulfonate (CHAPS), 0.03% cholesteryl hemisuccinate-tris salt (CHS), naloxone (1 μM), Leupeptin (5 μg/ml), PMSF (0.5 mM), 1X Halt protease inhibitor cocktail (ThermoFisher Scientific, 87786, Waltham, MA)], nutated for 1 hr at 4°C, CaCl 2 added to a final concentration of 1 mM, and then centrifuged at 100,000 x g for 1 hr, at 4°C. .. The resulting supernatant was then applied to an M1 anti-Flag column (Sigma, A4596, St. Louis, MO).

Article Title: Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
Article Snippet: .. Pellets from FMOR expressing HEK293 cells were solubilized in solubilization buffer [20 mM HEPES pH 7.5, 150 mM NaCl, 30% glycerol, 0.5% n-dodecyl-b-D-maltoside (DDM), 0.3% (3-((3-cholamidopropyl) dimethylammnonio) 1-propanesulfonate (CHAPS), 0.03% cholesteryl hemisuccinate-tris salt (CHS), naloxone (1 mM), Leupeptin (5 mg/ml), PMSF (0.5 mM), 1X Halt protease inhibitor cocktail (ThermoFisher Scientific, 87786, Waltham, MA)], nutated for 1 hr at 4 ̊C, CaCl2 added to a final concentration of 1 mM, and then centrifuged at 100,000 x g for 1 hr, at 4 ̊C. .. The resulting supernatant was then applied to an M1 anti-Flag column (Sigma, A4596, St. Louis, MO).

Protease Inhibitor:

Article Title: Structural Insights into Subunit-Dependent Functional Regulation in Epithelial Sodium Channels
Article Snippet: .. Then, 150μl of either DDM solubilization buffer (20 mM n-dodecyl-β-D-maltoside, 3 mM cholesteryl hemisuccinate, 200 mM NaCl, 20 mM HEPES, pH 7.4 and Thermo Fisher Halt Protease Inhibitor Cocktail at a volume ratio of 1:100) or digitonin solubilization buffer (7% digitonin, 200 mM NaCl, 20 mM HEPES pH 7.4 and Thermo Fisher Halt Protease Inhibitor Cocktail at a volume ratio of 1:100) was used to resuspend the washed pellet, which were then allowed to nutate at 4°C for 1 hour. ..

Article Title: Structural insights into subunit-dependent functional regulation in epithelial sodium channels.
Article Snippet: .. Then, 150ml of either DDM solubilization buffer (20 mM n-dodecyl-b-D-maltoside, 3 mM cholesteryl hemisuccinate, 200 mMNaCl, 20 mM HEPES, pH 7.4 and Thermo Fisher e2 Structure 33, 349–362.e1–e4, February 6, 2025 Halt Protease Inhibitor Cocktail at a volume ratio of 1:100) or digitonin solubilization buffer (7% digitonin, 200 mM NaCl, 20 mM HEPES pH 7.4 and Thermo Fisher Halt Protease Inhibitor Cocktail at a volume ratio of 1:100) was used to resuspend the washed pellet, which were then allowed to nutate at 4 C for 1 hour. ..

Article Title: Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
Article Snippet: .. Pellets from FMOR expressing HEK293 cells were solubilized in solubilization buffer [20 mM HEPES pH 7.5, 150 mM NaCl, 30% glycerol, 0.5% n-dodecyl-b-D-maltoside (DDM), 0.3% (3-((3-cholamidopropyl) dimethylammnonio) 1-propanesulfonate (CHAPS), 0.03% cholesteryl hemisuccinate-tris salt (CHS), naloxone (1 mM), Leupeptin (5 mg/ml), PMSF (0.5 mM), 1X Halt protease inhibitor cocktail (ThermoFisher Scientific, 87786, Waltham, MA)], nutated for 1 hr at 4 ̊C, CaCl2 added to a final concentration of 1 mM, and then centrifuged at 100,000 x g for 1 hr, at 4 ̊C. .. The resulting supernatant was then applied to an M1 anti-Flag column (Sigma, A4596, St. Louis, MO).

Concentration Assay:

Article Title: Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
Article Snippet: .. Pellets from FMOR expressing HEK293 cells were solubilized in solubilization buffer [20 mM HEPES pH 7.5, 150 mM NaCl, 30% glycerol, 0.5% n-dodecyl-b-D-maltoside (DDM), 0.3% (3-((3-cholamidopropyl) dimethylammnonio) 1-propanesulfonate (CHAPS), 0.03% cholesteryl hemisuccinate-tris salt (CHS), naloxone (1 mM), Leupeptin (5 mg/ml), PMSF (0.5 mM), 1X Halt protease inhibitor cocktail (ThermoFisher Scientific, 87786, Waltham, MA)], nutated for 1 hr at 4 ̊C, CaCl2 added to a final concentration of 1 mM, and then centrifuged at 100,000 x g for 1 hr, at 4 ̊C. .. The resulting supernatant was then applied to an M1 anti-Flag column (Sigma, A4596, St. Louis, MO).

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